In Elizabethan times, plays generally took place at 2pm in the afternoon, with the actors rehearsing the play in the morning and putting it on in the afternoon.
There wouldn’t be an interval and the play would run a lot faster than modern performances of Shakespeare plays. Often they would put on a different play every afternoon, unless they had a hit, where they would put on the same play for four or five days.
The accents by the actors of plays nowadays are done with in a modern English accent, but sometimes the Globe puts on a Shakespeare play in the original pronounciation that the actors which have spoken in the 16th century.
You can hear about what it would have sounded like in this YouTube clip: https://www.youtube.com/watch?v=gPlpphT7n9s